| Name | transfer factor (TF) |
| Synonyms | transfer factor (TF) Transfactor-likeprotein (Arabidopsis thaliana gene At2g01060) (9CI) |
| CAS | 253764-88-4 |
| Physical and Chemical Properties | Chemical properties are soluble, dialysable, ultrafiltable, and freeze-dried. The properties are stable at low temperature (-20 ℃), and the activity is still not lost after cryopreservation for more than 45 years. However, it is not heat-resistant and inactivated at 56 ℃ for 30min. Not destroyed by Rnase, Dnase, trypsin and lysozyme. The maximum wavelength of ultraviolet light absorption is 250-260nm. |
| Use | Use can promote the release of interferon. It is clinically used to treat diseases related to the weakening or deficiency of cellular immunity, such as leprosy, systemic vaccinia, Candida albicans infection, intracellular infection caused by Virus, bacteria or mold, malignant tumor, rheumatoid arthritis, etc. The injection site is often soreness, pain. Individual cases of rubella-like rash, skin itching. A small number have transient Fever. Chronic active hepatitis after oral administration of TF, occasionally liver function damage aggravated, and then gradually recovered. |
methods 1. dialysis
Wash the spleen, remove membrane, tendons and fat, wash and cut with cold redistilled water, add the same amount of distilled water, mash. After microscopic examination, most lymphocytes have been broken, freeze at -30 ℃, melt in water bath at 37 ℃, repeatedly freeze and thaw for 6-8 times, freeze and centrifuge for 20min, take the supernatant, and dialyze in refrigerator at 4 ℃ for 48h to obtain.
Methods 2. column chromatography
Spleen or tonsil, cut, copper sieve grinding, add normal saline to white fiber during grinding, freeze and thaw 4 times, centrifuge to take the supernatant, add dextran gel G-25 chromatography column, 20% the negative sulfosalicylic acid test as an indication, collect the active components to obtain.
Methods 3. ultrafiltration
Spleen or tonsils, cut and crush, add 2 times the amount of deionized water, mash with a masher at low temperature to prepare a homogenate, freeze at -30 ℃, melt in a water bath at 37 ℃, freeze and thaw again for 2-3 times, freeze and thaw again in liquid nitrogen and a water bath at 37 ℃, add 3 times the amount of cold ethanol (80%), remove protein precipitate, 0.1 mol/L, vacuum freeze drying, ethanol and moisture are removed to obtain light yellow concentrated solid, diluted with distilled water (1g of tissue plus 2ml of water), and pressurized filtration with ultrafiltration membrane (relative molecular mass above 12500 cannot pass) to obtain light yellow transparent liquid, I .e. TF.
| use | can promote the release of interferon. It is clinically used to treat diseases related to weakened or defective cellular immunity, such as leprosy, systemic vaccinia, Candida albicans infection, intracellular infections caused by viruses, bacteria or molds, malignant tumors, rheumatoid arthritis, etc. The injection site is often sore and painful. Some cases have rubella-like rash and skin itching. A few people have a brief fever. After oral administration of TF for chronic active hepatitis, liver function damage occasionally worsened and gradually recovered thereafter. |
| production method | method 1. dialysis method to wash the spleen, remove membrane, tendons and fat, wash and cut with cold heavy distilled water, add the same amount of distilled water, mash, after microscopic examination, freeze most lymphocytes at -30 ℃, melt in water bath at 37 ℃, freeze and thaw repeatedly for 6-8 times, freeze and centrifuge for 20min, and take the supernatant, TF can be obtained by dialysis at 4 ℃ for 48h. Methods 2. column chromatography of spleen or tonsil, cutting, copper sieve grinding, grinding, adding normal saline until white fiber, freeze and thaw 4 times, centrifugation to take the supernatant, dextran gel G-25 chromatography column, 20% sulfosalicylic acid test negative as an indication, collect the active components to obtain. Methods 3. the spleen or tonsil by ultrafiltration method, cut and crush, add 2 times the amount of deionized water, mash with a crusher at low temperature to prepare a homogenate, freeze at -30 ℃, melt in 37 ℃ water bath, freeze and thaw repeatedly for 2-3 times, freeze and thaw again in liquid nitrogen and 37 ℃ water bath, add 3 times the amount of cold ethanol (80%), remove protein precipitate, 0.1 mol/L hydrochloric acid to adjust pH 5.2, centrifuge for 30min to take the supernatant, freeze-dry in vacuum, remove ethanol and water to obtain light yellow concentrated solid, dilute with redistilled water (1g of tissue plus 2ml of water), and pressure filter with ultrafiltration membrane (relative molecular mass above 12500 cannot pass through) to obtain light yellow transparent liquid, I .e. TF. |